ABSTRACT
Objective To construct the male and female rat model of fluorosis and to explore the gender difference in fluorosis. Methods A total of 48 clean-grade SD rats with the body weight of about 150 g (male∶female=1∶1) were equally divided into the control group and fluorosis model group. Rats in the fluorosis model group were fed with fluorine-containing (100 mg/kg) feedstuff. The general condition, changes in body weight and dental fluorosis of the rats were observed every three days. A batch of female and male rats were sacrificed by femoral artery bleeding on the 70th and 110th day of experiment, respectively, when all rats showed dental fluorosis. The levels of urinary fluoride and bone fluoride of the rats were measured by a fluoride ion-selective electrode. Results All the male and female rats in the control group did not show dental fluorosis during the entire experiment. The female and male rats in the fluorosis group showed dental fluorosis visible to naked eye began approximately on the 60th and 80th day, respectively, indicating that the appearance of dental fluorosis of the male rats was about 20 days later than the female rats. After 30 days or so, the male and female rats went into a rapid growth period, and there were significant differences between the body weights of the male and female rats (P＜ 0. 01). The body weight of the male and female rats in the fluorosis group was higher than that of the control group on the 110th day. With the progression of fluorosis, the levels of urinary fluoride and bone fluoride of the rats were gradually increased, and finally significantly higher than those of the control group (P＜ 0. 01). There was a positive correlation between the levels of urinary and bone fluoride and the time of fluorosis. The levels of urinary and bone fluoride of the male rats were significantly higher than those of the female rats (P＜ 0. 01), indicating that they are affected by gender. Conclusions The body weight of the rat model of fluorosis, the starting time when dental fluorosis occurs and the levels of urinary and bone fluorine are all different between male and female rats.
ABSTRACT
Objective To explore the diagnostic values of procalcitonin(PCT),C-reactive protein (CRP),white blood cell(WBC),and neutrophil(N%)in bacterial infectious diseases. Methods Selected 272 patients with bacterial infectious diseases as the research object,all the patients were in accordance with diagnostic criteria of infection,and confirmed by laboratory examination and microbial cultures. Results The total sensitivity of bacterial infection was PCT > CRP > N% > WBC,and the specificity was PCT > CRP > WBC > N%. After different bacterial infection,klebsiella pneumonia was larger in view of PCT sensitivity than escherichia coli, followed by staphylococcus aureus,streptococcus pneumonia,and bauman acinetobacter in ranking. In view of CRP sensitivity,streptococcus pneumonia was larger than klebsiella pneumonia,followed by escherichia coli, staphylococcus aureus,and bauman acinetobacter in ranking. In respect of WBC sensitiveness,bauman acineto-bacter was larger than escherichia coli,followed by staphylococcus aureus,streptococcus pneumonia and klebsiella pneumoniae.In respect of N% sensitivity,streptococcus pneumonia was larger than klebsiella pneumonia,followed by bowman acinetobacter,escherichia coli,and staphylococcus aureus in ranking.After bacterial infection,PCT, CRP,WBC and N% were significantly higher than normal control group,and significantly higher than normal refer-ence value. After different bacterial infections,in view of the PCT level,klebsiella pneumonia was higher than streptococcus pneumonia,followed byescherichia coli,staphylococcus aureus,and bauman acinetobacter in rank-ing.In view of CRP level,klebsiella pneumonia was higher than streptococcus pneumonia,followed by escherichia coli,staphylococcal aureus and bowman acinetobacter. In view of WBC level,staphylococcus aureus was higher than bowman acinetobacter,followed by escherichia coli,klebsiella pneumonia and streptococcus pneumoniae. In view of N% level,klebsiella pneumonia was higher than streptococcus pneumonia,followed by bowman acineto-bacter,staphylococcus aureus and escherichia coli. Conclusion In terms of the sensitivity of PCT,CRP,WBC and NEC% to bacterial infection,the sequence is PCT>CRP>N%>WBC.